Day 1 (June 18th 9am – 1pm)
9:00 – 9:10: Welcome remarks from Christer Hogstrand & Thea Stewart:
9:10 – 9:20: Virtual introduction: Participant meet and greet.
Key Publications: Click to link to paper
9:20 – 9:40: Peter Kille (Cardiff University): Environmental Metallomics: The Key of Metal Chemistry
9:40 – 10:00: Sarah Fern (Imperial): SIMS and beyond – what Imperial has to offer.
10:00 – 10:20: Tina Geraki (I18 Diamond) and Julia Parker (I14 Diamond): What I18/I14 offers and key examples of biological sample analysis.
10:20 – 10:40: Stretch break & dance party and chat (BYOC – bring your own coffee)
10:40 – 11:00: Using an MMI Approach for Environmental Hazard Analysis: Tools, Tips and Tricks (Thea Stewart, London Metallomics Facility (LMF))
11:00 – 11:20: Matteo Minghetti (Oklahoma State): Sample preparation for x-ray fluorescent microscopy.
11:20 – 11:30: Plenary discussions: New horizons for metal imaging.
11:30 – 12:30: Break out groups – Multidimensional Metallomics: the burning biological questions, the technical challenges and potential of new insight.
12:30 – 13:00: Plenary: Question summary and Day 2 Homework.
Breakout Groups Summary
Key Discussion Points
- Importance of getting the sample preparation right to avoid artefacts whilst conducive to the requirements for each specific technique
- Requirements of appropriate standards and reference materials that can be used not only with one technique but the possibility of across techniques for quantitative bioimaging and determining LODs
- Establishing the correct sequence of experiments & analyses
- Determining appropriate methods of correlation between techniques, which may influence sample preparation
- Integrating contextualising imaging in the form of tagging or other morphological imaging approaches
- Addressing current inherent limitations in sample size/throughput
Day 2 (June 19th 9am – 1pm)
9:00 – 9:20: Day 2 Welcome remarks from Thea & Christer and program overview.
9:20 – 10:00: Break out session: Addressing metallomics questions: From ideas to action.
Explore how you would do multidimensional metallomics in three different contexts:
- Large organisms: Fish or large Mammal
- Small organisms or life stages: Nematode or fish egg (embryo)
- Individual Cells: Cultured cells or isolated cells
10:00 – 10:30: Plenary: Action summary
10:30 – 10:50: Stretch break & dance party and chat (BYOC – bring your own coffee)
10:50 – 11:20: Christer Hogstrand (KCL): Proposed pilot studies (zebrafish, earthworms and nematodes)
11:20 – 11:40: Professor Stephen Sturzenbaum (KCL) – Earthworm metal quantum dots and nematode metal handling.
11:40 – 12:30: Breakout groups:
- Technical group: Analytics and how to coordinate sample preparation for multidimentional metallomics.
- Biology group – Delivering biological insight.
12:30 – 12:50: Wayforward: Pilot experiments and wider engagement.
12:50 – 13:00: Christer Hogstrand (KCL) Close and Thanks