Multidimensional Metallomic Imaging for Environmental Hazard Analysis – Agenda

Day 1 (June 18th 9am – 1pm)

9:00 – 9:10: Welcome remarks from Christer Hogstrand & Thea Stewart: 

9:10 – 9:20: Virtual introduction:  Participant meet and greet.

Key Publications: Click to link to paper

Across the spectrum: integrating multidimensional metal analytics for in situ metallomic imaging – Theodora J. Stewart 2019

Analytical approaches to support current understanding of exposure, uptake and distributions of engineered nanoparticles by aquatic and terrestrial organisms – Schulz et al 2015

9:20 – 9:40: Peter Kille (Cardiff University):  Environmental Metallomics: The Key of Metal Chemistry

 

9:40 – 10:00: Sarah Fern (Imperial): SIMS and beyond – what Imperial has to offer.

 

 

10:00 – 10:20: Tina Geraki (I18 Diamond) and Julia Parker (I14 Diamond): What I18/I14 offers and key examples of biological sample analysis.

10:20 – 10:40: Stretch break & dance party and chat (BYOC – bring your own coffee)

 

10:40 – 11:00: Using an MMI Approach for Environmental Hazard Analysis: Tools, Tips and Tricks (Thea Stewart, London Metallomics Facility (LMF))

 

11:00 – 11:20: Matteo Minghetti (Oklahoma State): Sample preparation for x-ray fluorescent microscopy.

 

11:20 – 11:30: Plenary discussions: New horizons for metal imaging. 

11:30 – 12:30: Break out groups – Multidimensional Metallomics:  the burning biological questions, the technical challenges and potential of new insight. 

12:30 – 13:00: Plenary: Question summary and Day 2 Homework.

Breakout Groups Summary

Key Discussion Points

• Importance of getting the sample preparation right to avoid artefacts whilst conducive to the requirements for each specific technique
• Requirements of appropriate standards and reference materials that can be used not only with one technique but the possibility of across techniques for quantitative bioimaging and determining LODs
• Establishing the correct sequence of experiments & analyses
• Determining appropriate methods of correlation between techniques, which may influence sample preparation
• Integrating contextualising imaging in the form of tagging or other morphological imaging approaches
• Addressing current inherent limitations in sample size/throughput

Day 2 (June 19th 9am – 1pm)

9:00 – 9:20: Day 2 Welcome remarks from Thea & Christer and program overview.

9:20 – 10:00: Break out session: Addressing metallomics questions: From ideas to action.

Explore how you would do multidimensional metallomics in three different contexts:

• Large organisms: Fish or large Mammal 
• Small organisms or life stages: Nematode or fish egg (embryo)
• Individual Cells: Cultured cells or isolated cells

10:00 – 10:30: Plenary: Action summary

10:30 – 10:50: Stretch break & dance party and chat (BYOC – bring your own coffee)

10:50 – 11:20: Christer Hogstrand (KCL): Proposed pilot studies (zebrafish, earthworms and nematodes)

11:20 – 11:40: Professor Stephen Sturzenbaum (KCL) – Earthworm metal quantum dots and nematode metal handling.

11:40 – 12:30: Breakout groups:

• Technical group: Analytics and how to coordinate sample preparation for multidimentional metallomics.
• Biology group – Delivering biological insight.

12:30 – 12:50: Wayforward: Pilot experiments and wider engagement.

12:50 – 13:00: Christer Hogstrand (KCL) Close and Thanks